情况综述

产品

StemCell公司生产的人类胚胎干细胞/诱导多能干细胞 SSEA4正选试剂盒：EasySep hESC/hiPSC SSEA4 Positive Selection Kit

优点

EasySep试剂盒通过正选技术快速筛选出SSEA4阳性细胞，且是在无菌环境中进行，因此筛选出的阳性细胞可直接用于后续的细胞培养。

缺点

在分选SSEA4阳性细胞时所使用的抗体-磁珠复合体将始终附着在细胞表面，这也许会对这些分选出的细胞的某些下游的应用产生影响。

总结

如果你想分离诱导多能干细胞（iPSCs）或者纯化一定数量的多能干细胞，这种试剂盒不论是在分选的效率以及细胞活性的维持上都将是一种不错的选择！

Isolating cells from a heterogenous population is an important theme in stem cell biology. A number of cell isolation techniques are available to researchers; however, they are often expensive and can have disruptive effects on cell viability post-isolation.

The EasySep® SSEA4+ Kit is optimised for the positive selection the embryonic stem cell (ESC) marker SSEA4 and is very straightforward in its execution. The first step involves applying a SSEA4 selection cocktail to your cell suspension. This is followed by the addition of magnetic beads which subsequently attach to successfully labelled cells. The second stage involves a series of incubations of the magnetically labelled cell solution within a magnet. Upon inversion of the magnet, unlabelled cells are washed away while any labelled cells remain within the magnet. If conducted under sterile conditions the isolated cells can be successfully maintained post-isolation with little effect on cell viability. As the SSEA4 antigen is present on the surface of pluripotent stem cells, such as human ESCs and induced pluripotent stem cells (iPSCs), this system can be successfully used to isolate small numbers of iPSCs or alternatively to purify a population of hESCs.

I have been using this system for the past year on a monthly basis and have found this technique to be a reproducible and reliable way of isolating populations of pluripotent stem cells. The main advantages of this system are the efficiency of the isolation procedure with a high purity (98-99%) achieved post-isolation, as identified through FACS analysis. Also, the passive nature of this procedure allows isolated cells to be cultured without detrimentally affecting cell viability. Since the antibody-magnetic bead complex remains attached to the cells post-isolation, downstream applications could be affected; however, it did not appear to affect cell viability of hESCs post-isolation.

Overall, this system is an excellent way of isolating pluripotent stem cells based on the expression of the SSEA4 antigen.

Fiona Lewis

PhD Student

UK Centre for Tissue Engineering

University of Liverpool

United Kingdom

产品信息

STEMCELL公司北京办事处

STEMCELL Technologies Inc. Beijing Representative Office
Suite 1701-08 Hyndai Motor Tower, 38 Xiaoyun Road, Chaoyang District, Beijing, 100027, China
Tel: 10.64108515
Email: info.cn@stemcell.com

技术支持：

STEMCELL Technologies Inc.
570 West Seventh Avenue, Suite 400, Vancouver, BC, V5Z 1B3, Canada
Tel: 1.800.667.0322
Email: techsupport@stemcell.com

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