情况综述

产品

Life Technologies (Molecular Probes/Invitrogen)公司CellTrace™ CFSE 细胞增殖试剂盒 (CellTrace™ CFSE Cell Proliferation Kit)

优点

以方便的一次性瓶装形式提供用途广泛，稳定性高的细胞示踪剂。 羧基荧光素二醋酸盐琥珀酰亚胺酯，常称为 CFSE，可被动扩散进入细胞。 这种试剂没有颜色和荧光，直至细胞内的酯酶去除其醋酸盐基团，产生能发出明亮荧光的羧基荧光素琥珀酰亚胺酯。 琥珀酰亚胺酯与细胞内氨基反应，形成稳定且能用醛固定剂固定的荧光偶联物。 此试剂盒包含十份一次性瓶装的 CFSE，因此进行小规模实验时无需制备过量的易变质 CFSE 储存液。 此试剂盒还包括高质量的 DMSO（二甲基亚砜）和详细的实验方案。

缺点

如果使用CFSE进行细胞增殖检测是实验室常用技术，那最好单独购买CFSE。

总结

这是一款非常好的基于流式技术检测细胞增殖的试剂盒。十份一次性瓶装的 CFSE也会最大限度的减少CFSE长期保存变质对实验结果产生影响。

Flow cytometry is one of the most important tools in many scientific fields, including immunology. The ability to stain populations of cells and quantify these populations in a few hours has revolutionized the field of immunology. The identification of the complexity of the immune system is due in part to flow cytometry.
Besides labeling cells with various cell markers in order to identify the cells present in your sample, cells can also be labeled with carboxy-fluorescein diacetate, succimidyl ester, or CFSE, a small fluorescent dye. Although colorless outside of the cell, once a cellular esterase activates this compound, it will label your cells several logs above your control (brightness has been shown to be cell type specific). This dye is very photostable and thus allows for long term studies of cell proliferation, usually in the context of immunological cell activate (i.e., T cell proliferation after TCR receptor activation, etc.). Once the fluorescently labeled cells are manipulated and cultured (or put back into an animal), the fluorescent intensity will be diluted by approximately one half every time the cell divides, since two cells are now labeled instead of one. Several cell divisions can be observed in this assay (Some labs have seen up to eight generations).
Using this kit in our lab, we were able to measure cell proliferation easily without prior experience with the assay. We were only interested in a shorter time frame of 4 generations and did not try for the higher end of 8 generations. The reproducibility of ours results has been very robust. While the individual aliquots help keep our experiments straightforward and reproducible, they also add some cost to the assay. Our lab does not regularly perform cell proliferation experiments, but labs that do would be better off purchasing larger quantities of CFSE and troubleshooting the protocol with fresh solutions for each experiment.
Overall, this assay is robust and extremely straightforward. Until other companies turn to developing competing kits, Invitrogen’s kit is the only option, and it is well worth the money for most labs.

Jeffrey Perry

Lab Manager
Microbiology and Immunology
University of Michigan
United States

产品信息

联系方式

Invitrogen Beijing Office
No.7, Rong Chang East Street
Long Sheng Industry Park, Building 203 BDA
Beijing, 100176
P.R.C., CHINA
Phone:(86-10) 5808-5888
传真：(86-10) 6780-6535
电子邮件：Sales-cn@invitrogen.com

Invitrogen Corporation Shanghai Representative Office
Room 4010, Grand Gateway Tower 1
No.1 Hongqiao Road
Shanghai, 200030
P.R.C., CHINA
Phone:(86-21) 6145-2000
传真：(86-21) 6448-4077
电子邮件：Sales-cn@invitrogen.com

Invitrogen Guangzhou Office
Room 413, Haizhu Tech Park,
NO 2429 Xingangdong Road
Haizhu District, Guangzhou, 510320
P.R.C., CHINA
Phone:(86-20) 8923-2499
传真：(86-20) 8759-8763
电子邮件：Sales-cn@invitrogen.com