Roche公司PCR实验技术应用手册（第三版）
PCR Applications Manual - 3rd edition
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- Table of contens (PDF-File - 597 KB)
- Introduction (PDF-File - 611 KB)
- General Guidelines (PDF File - 528 KB)
- Primer design and template preparation (PDF-File - 1.00 MB)
- General PCR methods (PDF-File - 1.43 MB)
- General RT-PCR methods (PDF-File - 1.52 MB)
- Post-PCR Purification and Cloning (PDF-File - 1.51 MB)
- Real-Time PCR Methods (PDF-File - 1.21 MB)
- Applications (PDF-File - 1.44 MB)
- Appendix (PDF-File - 863 KB)
目录
1.1 Principles of PCR and RT-PCR
1.2 The Evolution of PCR
1.3 Purpose of this PCR Applications Manual
2.1 Preventing Contamination in the PCR Laboratory
2.2 Factors To Consider When Setting Up a PCR Laboratory
2.3 Typical Workflow for PCR/RT-PCR
3 Primer Design and Template Preparation
3.1 Primer Design
3.2 Template Preparation
3.3 Protocols for Isolation of Typical Templates
PCR Protocol Selection Guide
4.1 Basic PCR
4.2 High Fidelity PCR
4.3 Long Template PCR
4.4 Amplification of Difficult Templates
4.5 Guidelines for Optimizing PCR
4.6 Preventing Carryover
5.1 Factors to Consider in RT-PCR
5.2 Using Protector RNase Inhibitor
5.3 One-Step RT-PCR
5.4 Two-Step RT-PCR
6 Post-PCR Purification and Cloning
6.1 Purification of PCR Products
6.2 Cloning of PCR Products
7.1 Introduction
7.2 Real-Time PCR Assay Formats
7.3 Quantification Methods for Real-Time PCR
7.4 Product Characterization and Genotyping by Melting Curve Analysis
7.5 Real-Time PCR Instruments Available from Roche Applied Science
7.6 Real-Time PCR Reagents
7.7 Published Examples of Applications for the LightCycler® Carousel-Based Systems
General Introduction
8.1 Multiplex PCR Using the FastStart High Fidelity PCR System
8.2 Specific Amplification of Difficult PCR Products from Small Amounts of DNA Using FastStart Taq DNA Polymerase
8.3 FastStart Taq DNA Polymerase Is Ideally Suited for RT-PCR of Laser Captured Microdissected Material
8.4 Cloning of mRNAs and Rapid Screening by Direct Colony PCR with the FastStart PCR Master
8.5 FastStart High Fidelity PCR System Simplifies Study of Epigenetics and DNA Methylation
8.6 Analysis of DNA Methylation Patterns at the BRCA1 CpG Island
8.7 Comparison of Several Hot-Start Taq DNA Polymerases for Detection of Differentially Expressed Genes by GeneFishing
8.8 Transcriptional Organization of the O Antigen Biosynthesis Cluster in the GC-Rich Bacterium Burkholderia cenocepacia
8.9 Transcriptional Analysis of a Retroviral Vector System that Transfers Intron-Containing Genes
8.10 Quantification of BRCA1 Expression Levels with Standard Roche RT-PCR Reagents: A Sensitive Method for Detecting Low Amounts of Transcripts
8.11 Tailor-made Solutions Exemplified with the High-throughput 5´ RACE Kit
Troubleshooting
General Information
Ordering Information
Abbreviations
References
Index
Trademarks and License Disclaimers
