该视频主要介绍了如何使用GE的全自动蛋白纯化系统ÄKTA™ avant 来装填GE的AxiChrom空柱，下边是一些装填过程涉及到的主要步骤，详细装填过程中系统的设置等请点击下载最后的文档查看。
Packing small-scale AxiChrom columns
Packing AxiChrom 50 and 70 columns with ÄKTA avant 150
1 Introduction
This document is an Addendum to AxiChrom 50-100 User Manual, 28-9331-08, and describes packing of AxiChrom 50 and 70 columns with ÄKTA™ avant 150.
2 Specifications
Compatible systems
AxiChrom 50 and 70 columns are compatible with the ÄKTA avant 150 system and integrated in UNICORN control software with a predefined method for Intelligent Packing.
Packing flow rates
3 Connect AxiChrom columns to ÄKTA avant
Introduction
This chapter describes the basic configuration for connecting AxiChrom 50 and 70 columns to ÄKTA avant 150.
4 Create column type
Introduction
Before creating the Intelligent Packing method in UNICORN a column type needs to be created with the Column Handling tool. This type consists of:
• AxiChrom column hardware
• Medium
• Pressure/flow parameters
• Bed height
For more information on Column Handling, refer to the documentation for your UNICORN software.
5 Creating an Intelligent Packing method using ÄKTA avant 150
Introduction
This chapter contains some additional information to the chapter Intelligent Packing of the column in the AxiChrom 50, 70 and 100 columns User Manual where the Intelligent Packing wizard is presented for ÄKTApilot™ and ÄKTAexplorer™. This chapter describes how to create an Intelligent Packing method for ÄKTA avant 150 based on either GE approved media or custom media.
6 Preparing the slurry, priming and packing the column using ÄKTA avant
Follow the instructions in the AxiChrom 50, 70 and 100 columns User Manual to prepare the slurry, prime the column, pour slurry into the column and to pack the column. The UNICORN pages may differ slightly for ÄKTA avant 150 but the concept is the same as for ÄKTAexplorer and ÄKTApilot.
详细装填步骤请点击下载电子文档
