Roche公司推出这款细胞裂解试剂盒可以用于培养细胞的快速的高通量基因表达研究，细胞裂解产物用于后续的实时荧光定量PCR。显著提高细胞裂解速度和简便性，只需在室温下进行5分钟的一步裂解操作即可直接用于cDNA的合成。
The RealTime ready Cell Lysis Kit is designed for rapid, high-performance gene expression studies using cultured cells in two-step, real-time RT-PCR applications. The kit uses an advanced one-step protocol for lysing 3 to 30,000 cells within 5 minutes at room temperature. The resulting lysate can be used directly in cDNA synthesis, eliminating the time-consuming RNA purification step. Finally, the cDNA can be analyzed in real-time PCR experiments at any throughput (e.g., LightCycler® 480 Instrument, 96- and 384-well; LightCycler® 1536 Instrument, 1536-well).
Due to the user-friendly packaging and easy-to-use protocol, the RealTime ready Cell Lysis Kit can be easily adapted to all experimental and throughput requirements. In addition, the kit easily integrates into all manual and automated cell-based gene expression workflows.
产品优点:
* 使用方便：一步纯化。Increase convenience: Replace cumbersome multi-step RNA purification protocols with a simple one-step cell lysis procedure.
* 反应时间短：只需要5分钟。Improve time to result: Lyse samples in just 5 minutes at room temperature without further steps.
* 减少中间步骤：DNA酶的作用。Reduce intermediate steps: Obtain faster, high-quality results by performing DNase treatment during cDNA synthesis.
* 产品应用广泛：Benefit from broad applicability: Scale up from 3 to 30,000 cultured cells for real-time PCR applications in 96- to 1536-well formats with or without automation.
背景信息:
The RealTime ready Cell Lysis Kit is optimized when using the following downstream products for cDNA synthesis and real-time PCR analysis: Transcriptor cDNA Synthesis Kits, LightCycler® 480 DNA Masters, RealTime ready DNA Probes Masters, Universal ProbeLibrary probes, and RealTime ready assays.
| Product | RealTime ready Cell Lysis Kit |
|---|---|
| Company | Roche Applied Science |
| Price |
询价
|
| More Information | |
| Catalog Number | 06366821001 |
| Quantity | 1 kit 50 lysis reactions with a final reaction volume of 40 µl each |
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