现在的位置: 首页实验视频, 蛋白技术>正文
GE 标签蛋白柱上酶切小技巧:On Column Tag Removal
2013年06月29日 实验视频, 蛋白技术 评论数 2 ⁄ 被围观 3,687+


标签蛋白的表达是研究目标蛋白的一种重要办法。而这个可能会影响到蛋白结构和功能研究的蛋白标签往往需要被去除。该视频介绍了几种用于标签蛋白柱上酶切的试剂,以及在使用过程中的一些小技巧。

20130629143316

The expression of tagged proteins is the major source of proteins for research purposes. It is often recommended to remove the tag, which might otherwise interfere with protein function or interactions. This video will give you some examples of automated on-column tag removal with different cleavage agents.
www.gelifesciences.com/protein-purification

更多相关资料请点击如下链接下载

           Automated tag removal using ÄKTAxpress (Life Science News 18, p.6-7)P. Eklund, T. Frigård, T. Strömqvist, H. O. Andersson, M. Galin, E. Linde, L. Hedkvist, C. Markeland-Johansson, P. Liljedahl, A. Sjöberg, N. Pettersson, M. Nilsson, and J. A. Simon Amersham Biosciences, Uppsala, Sweden

  • File type: Adobe PDF
  • File size: 99,387 bytes
           Recombinant Protein Purification Handbook - Principles and Methods..................................................................................................................................... 5 Common acronymns and abbreviations .....................................................................................

  • File type: Adobe PDF
  • File size: 5,693,756 bytes
           ÄKTAxpress for tagged proteinsÄKTAxpress is a robust and easy-to-use system for multi-step purification of affinity-tagged proteins and antibodies. Purposedesigned hardware, intelligent software, and pre-packed media enable high protein purity and increases productivity.

  • File type: Adobe PDF
  • File size: 1,052,207 bytes
           Automated Multistep Purification of (His)6 and GST-tagged ProteinsAutomated multistep purifications of five affinity tagged proteins were performed using ÄKTAxpress™, a new chromatography system. The following purification results are presented: • One (histidine)6 -tagged protein using four different multistep protocols • F...

  • File type: Adobe PDF
  • File size: 690,922 bytes
           Purification of MBP-tagged and Strep II-tagged ProteinsRecombinant tagged proteins can be purified to high purity in one step using affinity chromatography. The tag can either be a peptide or a protein, and the choice of tag depends on target protein characteristics and the aim for purifying the protein. Tw...

  • File type: Adobe PDF
  • File size: 503,229 bytes
           Purification of Strep(II)-tagged proteins using new prepacked columnsPurification is done under physiological conditions and mild elution preserves the activity of the target protein. The small bead size of the medium (34 μm) allows elution in narrow peaks minimizing the need for further concentration steps. StrepTrap HP...

  • File type: Adobe PDF
  • File size: 304,600 bytes
           GST Gene Fusion SystemC. 2.5.1.18) mutant with Trp 214 replaced by Phe (W214F). The protein was expressed in HeLa cells, as reported in Raghunathan, S. et al. Crystal structure of human class mu glutathione transferase GSTM2-2. Effects of lattice packing on conformational he...

  • File type: Adobe PDF
  • File size: 3,089,438 bytes
           Automated On-Column Tag Cleavage and Multistep Purification of (His)6 and GST-Tagged Proteins...plate. Optimization of on-column tag cleavage Effect of protease concentration...was loaded per lane. Automatic tagremoval using ÄKTAxpress Two proteins...Optimized conditions for on-column tag cleavage with PreScission P...

  • File type: Adobe PDF
  • File size: 622,413 bytes
           ÄKTAxpress for tagged proteinsGeneric Application Error Test JSP (Item) Generic Application Error Error Type Error Message Key Message Originating Command 0 _ERR_CMD_CMD_NOT_FOUND User CMN3101E The system is unavailable due to "404". System Command not found: "404...

  • File type: Web Page
  • File size: 1,760 bytes


无觅相关文章插件,快速提升流量

×
腾讯微博