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如何跑SDS-PAGE胶
2013年07月08日 实验视频 评论数 1 ⁄ 被围观 5,208+


灌胶完成后怎么跑胶呢?视频教你7步完成

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Okay now that you know how to cast an SDS-PAGE gel, how exactly do you use it? Here, Dan shows you how to set up your apparatus to run SDS-PAGE — everything in 7 easy steps.

WAIT!!
Don’t have a gel? Watch this video on How to Make an SDS-PAGE gel.

Some important points to remember:
- short glass plate faces inwards
- remove the comb carefully
- load samples inside the wells, and do not over fill (usually 10uL of sample is loaded)
- fill the inner chamber to the TOP with buffer
- fill the BOTTOM of the outer chamber with buffer
- attach power supply: RED to RED and BLACK to BLACK

DONE!



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