现在的位置: 首页新产品>正文
GE推出新一代分子排阻预装柱 Superdex™ 200 Increase
2013年08月08日 新产品 评论数 2 ⁄ 被围观 7,510+


3-columns

GE 医疗生命科学部发布一款最新的Superdex™ 200 Increase预装柱,它是为小规模(最大样品体积为500ul)的制备纯化设计,分子排阻分子量范围为10 000~600 000。它是Superdex系列预装柱的另一产品线,其优势在于:

提高分辨率
- 更高的纯度
- 更可靠的分析结果

较少运行时间
- 相同分辨率运行时间较少一半
- 单位时间内更多次的运行

多种应用
- 针对不同分子宽广的分离范围
- 可以耐受苛刻的清洗,碱稳定性(pH1-14)好
 延长层析柱使用寿命
 不同样品可以使用同一根层析柱
- 三种包装形式,满足不同客户需求

现在我们提供三个产品可供选择:
 28-9909-44 Superdex 200 Increase 10/300 GL
 28-9909-45 Superdex 200 Increase 5/150 GL
 28-9909-46 Superdex 200 Increase 3.2/300

Three column formats to match your needs

Superdex 200 increase 10/300 GL

Superdex 200 Increase
10/300 GL

  • Small-scale preparative runs (mg)
  • High resolution analysis
    (25 – 500 µl)

Superdex 200 increase 5/150 GL

Superdex 200 Increase
5/150 GL

  • Purity check
  • Rapid screening
  • Small sample (4 - 50 µl) and buffer consumption

Superdex 200 Increase 3.2/300

Superdex 200 Increase
3.2/300

  • High resolution analysus
    (4 – 50 µl)
  • Microscale preparative runs (µl)

三种类型的预装柱技术参数如下:

Superdex 200 Increase 10/300 GL Superdex 200 Increase
5/150 GL
Superdex 200 Increase 3.2/300
Sample volume 25 - 500 µl 4- 50 µl 4 - 50 µl
Flow rate: Rec: 0.75 ml/min
Max: 1.8 ml/min
Rec: 0.45 ml/min
Max: 0.75 ml/min
Rec: 0.075 ml/min
Max: 0.15 ml/min
Max pressure
(over the packed bed):
4.0 Mpa
580 psi
5.0 Mpa
725 psi
4.5 Mpa
625 psi
Theoretical plates > 48 000 N/m > 42 000 N/m > 48 000 N/m
Bed volume 24 ml 3 ml 2.4 ml
Recommended ÄKTA systems ÄKTApure, ÄKTAmicro, ÄKTApurifier 10, ÄKTAexplorer 10, ÄKTA avant 25 ÄKTApure, ÄKTAmicro, ÄKTApurifier 10 ÄKTApure, ÄKTAmicro, ÄKTApurifier 10,
Code no:28-9909-44 Code no: 28-9909-45 Code no: 28-9909-46

 

Superdex. Increased.

 

Superdex Increased

 

Superdex Columns

Superdex 200 Increase

The future of high-resolution size exclusion (gel filtration) chromatography— Increased performance with shorter run times

  • Half the run time* with same resolution
  • Increased resolution* for higher purity and better analytical results
  • Versatile use: broad fractionation range, alkaline stable for cleaning, preparative and analytical uses

*compared with Superdex 200

 

技术细节:
Superdex 200 Increase 与传统的Superdex200 相比,前者使用更高流速,耐压性能更好的8.6um 颗粒大小的交联
琼脂糖和葡聚糖为基架。两者的分离范围一致。

Superdex 200 Superdex 200 Increase
Matrix Cross-linked agrose and dextran
Fractionation range Molecular weights from 10 000 to 600 000
Flow/pressure properties Regular Rigid beads with high flow/pressure resistance
Average bead size 13µm 8.6 µm
pH stability
Regular use
Short term
pH 3 - 12
pH 1 - 14

 

Superdex 200 Increase

Versatile, rapid protein separation and high resolution results

Superdex 200 Increase size exclusion chromatography columns are designed for both preparative and analytical purposes for a broad range of biomolecules, enabling higher purity, and improved protein analysis. The column delivers up to double the resolution in the same time* or the same resolution in a half of the time*, depending on the conditions selected and the molecule (*compared with Superdex 200).

Optimized for MAb separations, Superdex 200 Increase prepacked columns can be used for small-scale (µg-mg) high resolution size exclusion chromatography and for characterizing a variety of different proteins with molecular weights between 10 000 – 600 000. The high-flow agarose base matrix is pH stable and easy to clean.  In addition, its low nonspecific interaction provides for long column life.

Superdex 200 Increase, available in three sizes (10/300 GL; 5/150 GL and 3.2/300 GL), can be used with ÄKTA chromatography systems for a variety of applications including protein purification, aggregate analysis, studies of complex formation, and sample screening.  All three columns can also be used on different HPLC systems.
*compared with Superdex 200

Superdex 200 Increase 10/300 GL

Application Note
Size exclusion chromatography analysis of papain-cleaved monoclonal antibody using Superdex 200 Increase columns Summary

This application note describes a simplified workflow for the production of Fab (fragment, antigen binding). Fab was produced by papain cleavage of a purified monoclonal antibody (MAb) followed by purification on HiTrap MabSelect SuRe and HiTrap Protein L columns. Intermediates and end products were analyzed with size exclusion chromatography (SEC, also called gel filtration) on two different sized Superdex 200 Increase columns: one 300 mm and one 150 mm in length. The high resolution power of Superdex 200 Increase allows baseline separation between antibody monomer, aggregates, and fragments.Close
Data File
Superdex 200 Increase columns Summary

Superdex 200 Increase prepacked columns are designed for size exclusion chromatography (SEC)/high resolution gel filtration in small-scale (µg to mg), preparative purification, as well as for characterization and analysis of proteins with molecular weights (Mr) from 10 000 to 600 000. These versatile columns offer rapid separations with high resolution for a variety of applications including protein purification, aggregate analysis, studies of complex formation, and screening of samples. Superdex 200 Increase columns offer: versatile use for both preparative and analytical purposes, for a large variety of proteins; increased resolution compared with Superdex 200, for higher purity and improved analysis results; and reduced runtime compared with Superdex 200, to get results faster.Close
Instruction/Protocol
Superdex 200 Increase 10/300 GL & 5/150 GL Summary

Superdex 200 Increase 5/150 GL is for rapid size analysis of proteins and other biomolecules. Short cycle times, together with small sample volume and low consumption of buffer make this column suitable for use in screening experiments to check protein homogeneity. Superdex 200 Increase 10/300 GL is suitable for small scale preparative purification (μg-mg) as a final polishing step, as well as for protein analysis and characterizationClose

 

更多产品细节和应用信息见
http://www.gelifesciences.com/newsec
更多相关信息,请 咨询GE医疗生命科学部热线:800-810-9118



无觅相关文章插件,快速提升流量

×
腾讯微博