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A presentation showing how hESC derived cardiomyocytes can be a powerful approach to assessing cardiac drug liabilities. Nick Thomas describes how hESC derived Cytiva Cardiomyocytes: • are a valid model of cardiomyocytes from the human heart • are compatible with assays routinely used for testing cardiotoxicity • together with high-content assays and analysis, can indicate the mechanism of toxicity of drug compounds http://www.gelifesciences.com/cardiomyocytes Cytiva Car...
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生物库——带你畅游生命科学的海洋!

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2011年12月06日 讲座课堂 ⁄ 被围观 6,666+
讲座题目:Quantitative Western blotting - Webinar 讲座人:Maria Winkvist Ph.D GE Healthcare Bio-sciences AB Uppsala, Sweden Western Blot中文一般称为蛋白质印迹。它是分子生物学、生物化学和免疫遗传学中常用的一种实验方法。其基本原理是通过特异性抗体对凝胶电泳处理过的细胞或生物组织样品进行着色。通过分析着色的位置和着色深度获得特定蛋白质在所分析的细胞或组织中的表达情况的信息。 Western Blot与Southern或Northern杂交方法类似,但Western Blot采用的是聚丙烯酰胺凝胶电泳,被检测物是蛋白...
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Lecture 8 - Cell Communication and Immunology (cont.) 课程摘要 Professor Saltzman continues his discussion of cell communication in the body, extending the description to the nervous and immune system. Professor Saltzman describes the mode of signal transmission in neurons: action potential in the axon, and neurotransmitter release at the synaptic cleft. He also introduces elements of the innate and adaptive immune system. The adaptive immune system is presented as a host/...
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Lecture 7 - Cell Communication and Immunology 课程摘要 Professor Saltzman talks about cell communication, specifically ligand-receptor interactions that are important in maintaining homeostasis in the body. Different types of receptors and ligands, the nature of their interactions and ways to apply this into developing drugs are discussed (eg. Aldopa, Taximofen, beta-blockers). Next, Professor Saltzman talks about kinases, phosphatases, cyclic AMP and the mechanism of swit...
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Lecture 6 - Cell Culture Engineering (cont.) 课程摘要 Professor Saltzman describes the processes of fertilization and embryogenesis. Professor Saltzman then talks about the definition and classification of different types of stem cells, where stem cells are found in the body, and the potential for use of stem cells in treating diseases. Some challenges in this type of therapy are also discussed. Finally, Professor Saltzman introduces the exponential equation for cell growt...
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Lecture 5 - Cell Culture Engineering 课程摘要 Professor Saltzman reviews the concept of gene therapy, and gives some examples of where this is applied. Methods to help deliver DNA into cells using viruses and cationic lipids are discussed, as a way to overcome some challenges in gene therapy. Next, Professor Saltzman gives a brief introduction into bacterial and mammalian cell physiology. He describes the different tissues in the body, the cell development/differentiation ...
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Lecture 4 - Genetic Engineering (cont.) 课程摘要 Professor Saltzman continues his presentation on DNA technology by discussing control of gene expression using two methods of RNA silencing: anti-sense therapy and RNA interference. Molecular cloning techniques to mass-produce proteins using plasmid, restriction enzymes, ligase, and antibiotic selection in bacteria are discussed. Steps and molecules involved in polymerase chain reaction are also described. Professor Saltzman...
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Lecture 3 - Genetic Engineering 课程摘要 Professor Saltzman introduces the elements of molecular structure of DNA such as backbone, base composition, base pairing, and directionality of nucleic acids. He describes the processes of DNA synthesis, transcription, RNA splicing, translation, and post-translational processing required to make a protein such as insulin from its genetic code (DNA). Professor Saltzman describes the genetic code. RNA interference is also discussed a...
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Lecture 2 - What Is Biomedical Engineering? (cont.) 课程摘要 Class begins with discussion of students' answers to the two questions given as assignment in the previous lecture. Professor Saltzman talks about the basic concept of biomedical engineering and two separate aspects of it: gaining better understanding of human physiology and developing ways to improve human health. He then introduces the term homeostasis, and talks about parameters that are involved in controlli...
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Lecture 1 - What Is Biomedical Engineering? 课程摘要 Professor Saltzman introduces the concepts and applications of biomedical engineering, providing an overview of the course syllabus, reading materials for lecture and labs and grading logistics. Various pictures are shown to highlight the current application of biomedical engineering technologies in daily life (eg. chest x-ray, PET scan, operating room, gene chip, transport). Next, living standards and medical technologi...
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