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2015年10月30日 分子技术 ⁄ 被围观 485+
著作权归作者所有。 商业转载请联系作者获得授权,非商业转载请注明出处。 作者:林宇翔 链接:http://www.zhihu.com/question/22356523/answer/21108527 来源:知乎 CRISPR ; 规律成簇的间隔短回文重复(Clustered Regularly Interspaced Short Palindromic Repeats),是大多数细菌及古细菌中的一种天然免疫方式。 现在使用的CRISPR/cas 9系统是由最简单的type II CRISPR改造而来,该系统由单链的guide RNA和有核酸内切酶活性的Cas 9蛋白构成。下图解释其机理:(RE C, SCIE E O F, SOU E. The CRISPR craze[J]....
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生物库——带你畅游生命科学的海洋!

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2015年10月26日 细胞研究 ⁄ 被围观 371+
GE Healthcare Life Sciences offers handbooks covering a wide range of techniques and applications including sample preparation, protein purification, electrophoresis, blotting, and cell culture. Whether you’re a beginner or an expert, our handbooks are a great reference for practical tips and in-depth information about common methodologies used in the lab. Our handbooks provide theory, glossaries, workflows, protocols, recipes, and even troubleshooting guides to help ma...
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2015年10月26日 分子技术 ⁄ 被围观 332+
GE Healthcare Life Sciences offers handbooks covering a wide range of techniques and applications including sample preparation, protein purification, electrophoresis, blotting, and cell culture. Whether you’re a beginner or an expert, our handbooks are a great reference for practical tips and in-depth information about common methodologies used in the lab. Our handbooks provide theory, glossaries, workflows, protocols, recipes, and even troubleshooting guides to help ma...
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2015年10月26日 蛋白技术 ⁄ 被围观 347+
GE Healthcare Life Sciences offers handbooks covering a wide range of techniques and applications including sample preparation, protein purification, electrophoresis, blotting, and cell culture. Whether you’re a beginner or an expert, our handbooks are a great reference for practical tips and in-depth information about common methodologies used in the lab. Our handbooks provide theory, glossaries, workflows, protocols, recipes, and even troubleshooting guides to help ma...
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2015年10月26日 蛋白技术 ⁄ 被围观 313+
GE Healthcare Life Sciences offers handbooks covering a wide range of techniques and applications including sample preparation, protein purification, electrophoresis, blotting, and cell culture. Whether you’re a beginner or an expert, our handbooks are a great reference for practical tips and in-depth information about common methodologies used in the lab. Our handbooks provide theory, glossaries, workflows, protocols, recipes, and even troubleshooting guides to help ma...
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2015年10月26日 蛋白技术 ⁄ 被围观 614+
GE Healthcare Life Sciences offers handbooks covering a wide range of techniques and applications including sample preparation, protein purification, electrophoresis, blotting, and cell culture. Whether you’re a beginner or an expert, our handbooks are a great reference for practical tips and in-depth information about common methodologies used in the lab. Our handbooks provide theory, glossaries, workflows, protocols, recipes, and even troubleshooting guides to help ma...
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Gel filtration has successfully been employed for size based separations of macromolecules since the late 1950s by the introduction of the first commercially available medium (resin), Sephadex, in 1959. Gel filtration, or size exclusion chromatography, may be performed mainly in two principally different modes, depending on the size differences of the solutes to be separated: group separation and fractionation. The main usage for Sephadex product family is group separation...
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2015年09月10日 分子技术 ⁄ 被围观 2,510+
感谢: 百奥赛图 的供稿. CRISPR/Cas9技术原理简介 最近几年基因编辑技术异常火爆,CRISPR/Cas9技术面世以后,弥补了传统基因编辑技术的诸多不足,使得基因的“任意编辑”变得越来越容易。也因此,CRISPR/Cas9技术当仁不让的成为基因编辑技术的“王牌”,大有一副取而代之的势头。所以这里就给大家简单介绍一下CRISPR/Cas9的技术原理! 一、CRISPR/Cas9系统的构成 CRISPR(clustered,regularly interspaced,short palindromic repeats)是一种来自细菌降解入侵的病毒DNA或其他外源DNA的免疫机制。在细菌及古细菌中,CRI...
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  点击下载:GE凝胶选择指南2015 目录 手动纯化产品 凝胶过滤 离子交换 标签蛋白纯化 抗体纯化 其他亲和纯化 疏水及反相纯化 工艺开发用层析柱 层析空柱及对应填料 附录一 层析柱相关接头 附录二 层析应用指南   此书中常用缩写 柱子类型常用缩写解释: ST:不锈钢柱。 PE:Peek材质,Tricorn柱形式。 GL:玻璃材质, Tricorn柱形式。 产品名常用缩写解释: XL:高载量。 Pg:Prep grade,制备级。 HR:High Resolution,高分辨率。 LS:Low Sub,低取代,配基密度低。 HS:High Sub,高取代,配基密度...
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感谢: 韩娟 的供稿. 基因敲除技术是自80年代末发展起来的一种导致特定基因失活或缺失的新型分子生物学技术。通常意义上的基因敲除主要是应用DNA同源重组原理,用外源片段替代靶基因片段,从而达到靶基因敲除的目的。随着基因敲除技术的发展,除了基于胚胎干细胞的同源重组方法外,新的技术不断涌现,例如:ZFN、TALEN、CRISPR/Cas9这些新兴技术使基因敲除的应用突破了物种的限制,几乎可以实现所有物种的各种编辑需求。下面给大家介绍一下目前应用最为广泛的三大基因敲除技术: 基于胚胎干细胞的同源重组技术——...
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