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2013年03月08日 动物技术 ⁄ 被围观 3,778+
感谢: 刘衣男 的供稿. TALEN(Transcription Activator-Like Effector Nuclease) 是一种人工改造的限制性内切酶,是由一个DNA识别域和一个核酸内切酶的切割域(FokⅠ)构成。DNA识别域是由一些非常保守的重复氨基酸序列模块组成,每个模块一般由34个氨基酸(aa)组成,其中第12,13位氨基酸种类可变且决定了该模块识别靶向位点的特异性。通过DNA识别域结合到靶位点上,FokI的切割域形成二聚体,可以特异性的切断目标基因,损伤后的DNA在非同源末端连接修复过程中,会由于碱基的随机增减造成目标基因功能缺失。 由...
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Optimized Fibrin Gel Bead Assay for the Study of Angiogenesis Martin N. Nakatsu, Jaeger Davis, Christopher C.W. Hughes Department of Molecular Biology and Biochemistry, University of California, Irvine 摘要 Angiogenesis is a complex multi-step process, where, in response to angiogenic stimuli, new vessels are created from the existing vasculature. These steps include: degradation of the basement membrane, proliferation and migration (sprouting) of endothelial cells (EC) ...
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鸡胚绒毛尿囊膜(chick embryo chorioallantoic membrane, CAM)技术,简称鸡胚模型(CAM) 理论基础: 通常情况下,除了胚胎发育和女性月经周期外,机体新血管的形成,很少发生;但在病理情况下,如损伤治愈、炎症、糖尿病性视网膜病变、银屑病及硬皮病等都有血管生成,特别是实体肿瘤的生长和转移与血管生成密切相关。因此,抑制血管生成可能是抗肿瘤生长和转移的有效途径。建立各种体内血管生成模型及体外检测与血管生成有关的内皮细胞的各项指标是实现寻找新的抗血管生成药物的关键。 基本原理: 鸡胚正常血管...
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2011年09月11日 动物技术 ⁄ 被围观 9,116+
Aorta Ring Assay A new approach of the molecular genetics of angiogenesis Introduction Angiogenesis, the development of new blood vessels from preexisting ones, is an essential feature of tissue remodelling and wound healing. Consequently, extensive interests are generated in the molecular mechanism elucidation of vessel growth. The angiogenic process is regulated both by positive and negative factors that modulate the migration, proliferation, proteolytic activity and di...
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2011年09月11日 动物技术 ⁄ 被围观 6,477+
Protocol for evaluating vascular permeability in wildtype and PRTg mice Tom Murphy Created: 9/14/01 1) Female Tiel-PR Tg mice and wildtype control litter-mates aged 6-9 wks were ovariectimized and allowed to recover for 7-10 days. Making estrogen and progesterone stock solutions: For estrogen (17 beta-estradiol from Sigma), weigh out 10 mg and add to 1 ml 200-proof EtOH. This will yield a lOmg/ml solution. Mix by vortexing. Perform a 1:100 dilution by adding 5 μl of the l...
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